Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Simple, Affordable Distance-Based Paper Analytical Device for the Quantitative Perfluorooctane Sulfonate Detection in Drinking Water and Beverage Samples.

ACS sensors·2026

Same author

Epitope-Imprinted Polymers for Selective Recognition of Bacterial Proteins: From Design to Analytical Applications.

ACS measurement science au·2026

Same author

Fully 3D-Printed Sampling-to-Detection Electrochemical Platform for Point-of-Care Measurement of Salivary Uric Acid.

ACS measurement science au·2026

Same author

Acceptor-phase-free gel electromembrane extraction integrated with smartphone colorimetry for the quantification of cephalexin in pharmaceutical and environmental water samples.

Talanta·2026

Same author

Sustainable high-throughput microwell spectrophotometric methods for avapritinib quality control via charge-transfer complexation.

Scientific reports·2026

Same author

Electrochemical Biosensors for Hepatocellular Carcinoma: Advances, Analytical Performance, and Clinical Translation.

Critical reviews in analytical chemistry·2026

Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026

Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026

Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026

Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026

Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026

Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 8, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

96-Well Agarose-Gel Electromembrane Extraction.

Thidarat Samkumpim^1,2, Samira Dowlatshah^1,3, Waleed Alahmad²

¹Department of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway.

Analytical Chemistry

|December 22, 2025

Summary

This summary is machine-generated.

A novel 96-well gel electromembrane extraction (EME) system using agarose gel membranes offers efficient and green extraction of polar pharmaceuticals from complex samples like human plasma.

More Related Videos

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases

Published on: August 1, 2025

Related Experiment Videos

Last Updated: Jan 8, 2026

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases

Published on: August 1, 2025

Area of Science:

Analytical Chemistry
Separation Science
Biotechnology

Background:

Traditional electromembrane extraction (EME) often utilizes oil membranes, presenting limitations in selectivity and environmental impact.
Developing more efficient and greener extraction methods is crucial for pharmaceutical analysis and sample preparation.

Purpose of the Study:

To demonstrate the first 96-well gel electromembrane extraction (EME) system utilizing agarose gel membranes.
To evaluate the efficiency and selectivity of this novel system for extracting pharmaceutical compounds from aqueous and plasma samples.

Main Methods:

Immobilization of 3% w/v agarose gel membranes (3.5 mm thickness) in polyvinylidene fluoride (PVDF) filters within a 96-well plate.
Extraction of 90 basic pharmaceuticals (log P range -4.2 to 8.1) from 200 μL samples (water or human plasma) into 200 μL of 100 mM formic acid acceptor.
Application of 25 V extraction potential for 20 min to minimize Joule heating and electroosmosis.

Main Results:

High recovery (40-100%) achieved for compounds within the polarity range -4.0 < log P < 3.0.
Discrimination observed for compounds with log P > 3.0 due to gel membrane interactions.
Efficient cleanup from human plasma samples, with no extraction of proteins and phospholipids.
Selectivity controlled by interactions with the aqueous gel membrane, differing from oil-based EME.

Conclusions:

The 96-well agarose-gel EME system demonstrates significant potential for efficient and selective extraction of polar analytes.
This gel-based EME approach offers a greener and more efficient alternative to traditional oil-based EME systems.
The system provides effective sample cleanup, making it suitable for complex matrices like human plasma.