Coronavesicle-engineered hydrogel sequential photothermal-immunometabolic therapy reprograms tissue microenvironment
Bodeng Wu1,2, Shan Lin1,2, Qi Xiu1,2
1Department of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Provincial Key Laboratory of Single-cell and Extracellular Vesicles, Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, P. R. China.
Abstract:
Persistent infection and dysregulated tissue microenvironment constitute the primary obstacles hindering the healing of infectious diabetic wounds. Herein, we developed an NIR-responsive hydrogel dressing incorporating polyphenol-engineered plant-derived nanovesicles (termed Coronavesicles) via an in-situ deposition strategy, designed to implement sequential photothermal-immunometabolic therapy for infectious diabetic wounds. The coronavesicles within the hydrogel preferentially adhere to bacterial surfaces to deliver localized photothermal therapy (PTT), wherein subsequent heat-triggered rupture releases intrinsic bioactive constituents to enhance antibacterial efficacy. Following bacterial clearance, the released components from coronavesicles synergistically modulate fibroblast proliferation, alleviate oxidative stress, and reverse macrophage-mediated inflammatory immunity. This multi-pronged therapeutic strategy demonstrates desirable treatment outcomes both in vitro and in vivo. This work contributes to the understanding and future development of engineering plant-derived nanovesicles for diabetic wound management.
