Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.2K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.2K
Labeling DNA Probes03:31

Labeling DNA Probes

9.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.2K
Immunoprecipitation01:20

Immunoprecipitation

6.7K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coronavirus membrane protein with a fluorescent protein tag enables particle tracking for the study of virus assembly and egress in live cells.

Journal of virology·2026
Same author

Energy-translation coupling limits anaerobic yeast growth.

Nature communications·2026
Same author

A Prodrug Strategy to Conditionally Trap Therapeutic Payloads for Improved Tumor Retention.

ACS central science·2026
Same author

Design and commissioning of a new synchrotron beamline dedicated to X-ray footprinting mass spectrometry.

Journal of synchrotron radiation·2026
Same author

Recent Advances in Graphene-Based Field-Effect Transistor Biosensors for Disease Biomarker Detection and Clinical Prospects.

Biosensors·2026
Same author

Synthetic Control over the Electron-Beam Stability of Upconverting Nanoparticles.

Nano letters·2026

Related Experiment Video

Updated: Jan 8, 2026

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
06:48

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

Published on: September 15, 2016

12.3K

Precision Labeling of Native Antibodies with Lock Coupling.

Yazhi Liu1,2, Isha Nadig1,3, Abijeet Singh Mehta2,4

  • 1The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.

Journal of the American Chemical Society
|December 23, 2025
PubMed
Summary
This summary is machine-generated.

Lock coupling creates stable protein bonds using a simple reaction between lysine and acidic amino acids. This method precisely labels antibodies for advanced applications in bioimaging and nanomedicine.

More Related Videos

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
09:06

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Published on: December 23, 2016

22.1K
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
07:48

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

9.2K

Related Experiment Videos

Last Updated: Jan 8, 2026

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
06:48

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

Published on: September 15, 2016

12.3K
Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
09:06

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Published on: December 23, 2016

22.1K
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
07:48

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

9.2K

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Biotechnology

Background:

  • Stable protein complexes are crucial for biotechnology but can dissociate, limiting applications.
  • Existing methods for protein modification can be complex or lack precision.

Purpose of the Study:

  • To introduce a simple, selective, one-step reaction for forming stable isopeptide bonds between proteins.
  • To develop a method for precise labeling of native antibodies for advanced imaging and biomaterial applications.

Main Methods:

  • Developed 'lock coupling,' a reaction between interfacial lysine and glutamate/aspartate side chains using EDC catalyst.
  • Optimized reaction conditions (pH, reagent addition order) to minimize nonspecific cross-linking.
  • Engineered GB1 protein for antibody Fc domain attachment and introduced cysteine for facile conjugation of probes (fluorophores, nanocrystals).

Main Results:

  • Demonstrated successful formation of stable isopeptide bonds for precise native antibody labeling.
  • Showcased uniform attachment of a defined number of probes to antibodies without extensive purification.
  • Validated labeled antibodies in live-cell imaging, serving as a stable alternative to secondary antibodies for multicolor immunostaining.

Conclusions:

  • Lock coupling offers a versatile and efficient method for protein modification and precise probe synthesis.
  • This technique has broad potential for developing advanced protein-based probes for imaging, biomaterials, and medicine.
  • The method leverages natural protein interactions and widespread amino acid pairings for robust bioconjugation.