[Analysis of soluble antigens of the human striated musculature. II. Immunochemistry studies]

Human skeletal muscle was extracted at pH 7 and the supernatant of entrifugation at 100 000 X g for 1h examined with different immunological methods. In these studies the following anti-sera absorbed with human serum proteins were used: anti-muscle, anti-heart, anti-brain, anti-kidney, and anti-pancreas serum. Further anti-muscle serum absorbed with human serum and liver extract was applied. At least 10 antigens typical for skeletal muscle could be detected with these different sera. One of the antigens with great probability is specific for skeletal muscle. Its electrophoretical mobility was 3,75-105cm2/V sec. Two antigens revealed enzymatic activities.