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Updated: Jul 21, 2026
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
Published on: October 16, 2015
[Rapid Analysis of Cyanide Based on a Ratiometric Fluorescent Probe Using Gold Nanoclusters-Fluorescein]
Tai-Shen He1, Zhong-Jiang Lü1, Yi-Ming Sun1
1West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objectives:
To establish a rapid analysis method for cyanide based on a ratiometric fluorescent probe, providing a quantitative strategy for on-site visual and rapid detection of cyanide.
Methods:
A dual-emission ratiometric fluorescent probe (AuNCs-FL) was constructed by using bovine serum albumin (BSA)-stabilized gold nanoclusters (AuNCs, fluorescence emission at 660 nm) as the responsive signal unit and fluorescein (FL, emission at 515 nm) as the internal reference.
Results:
The etching effect of cyanide on AuNCs resulted in fluorescence quenching at 660 nm, while the fluorescence intensity of FL at 515 nm remained unchanged, enabling a rapid response analysis of cyanide shift from red to green fluorescence. The developed probe enabled rapid analysis of cyanide within 3 min, with a limit of detection (LOD) of 3.4 mg/L and a visual detection range of 10-100 mg/L.
Conclusions:
The AuNCs-FL fluorescent probe is structurally simple, low-cost, and easy to operate, delivering rapid and accurate results. It also avoids the interference from sulfides encountered in commercial cyanide test kits, making it suitable for the on-site rapid detection of suspected powder samples in cyanide poisoning cases.
