Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

3.0K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
3.0K
Generation of Straight or Branched Actin Filaments01:14

Generation of Straight or Branched Actin Filaments

3.6K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
3.6K
Formation of Higher-order Actin Filaments01:11

Formation of Higher-order Actin Filaments

3.5K
The polymerization of G-actin monomers into filamentous F-actin is a multi-step process. Once the F-actins are formed, they can bundle together in different arrangements to form higher-order networks and regulate cellular functions. Common examples include the formation of lamellipodia and filopodia at the cell's leading edge by actin reorganization in a migrating cell. The microvilli on the brush border epithelial cells are also formed through the F-actin network.
The high-order actin...
3.5K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

3.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adrenergic nerves regulate B cell responses to large antigens through modulation of lymph node permeability.

Research square·2026
Same author

A Non-Canonical Role for Hepatocyte MLKL in Promoting Mitochondrial Dysfunction and Senescence in the Aging Liver.

Aging cell·2026
Same author

Unconventional activation of the proto-oncogene FGFR1 by extracellular phosphate via H<sub>2</sub>O<sub>2</sub>-mediated kinase oxidation.

Cell reports·2026
Same author

A Non-canonical Role for Hepatocyte MLKL in Promoting Mitochondrial Dysfunction and Senescence in the Aging Liver.

bioRxiv : the preprint server for biology·2025
Same author

P-glycoprotein expression skews mitochondrial dye measurements in T cells.

Frontiers in immunology·2025
Same author

Combining In Vivo 2-Photon Imaging with Photoactivatable Fluorescent Labeling Shows Low Rates of Mitochondrial Dynamics in Skeletal Muscle.

Medicine and science in sports and exercise·2025

Related Experiment Video

Updated: Jan 8, 2026

Purification of the Dendritic Filopodia-rich Fraction
11:51

Purification of the Dendritic Filopodia-rich Fraction

Published on: May 2, 2019

5.6K

A role for Fis1 in dendritic development.

Klaudia Strucinska1, Parker Kneis1,2, Travis Pennington1,3

  • 1Aging & Metabolism Program, Oklahoma Medical Research Foundation Aging & Metabolism Program, 825 NE 13th Street, MS21, Oklahoma City, OK, 73104, USA.

Scientific Reports
|December 23, 2025
PubMed
Summary

Fission 1 (Fis1) protein regulates mitochondrial size and dynamics in neuronal dendrites. Loss of Fis1 impacts neuronal calcium handling and connectivity, highlighting its crucial role in dendritic mitochondrial health.

More Related Videos

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons
10:13

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons

Published on: February 10, 2016

12.7K
Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila
07:13

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

Published on: January 7, 2019

14.6K

Related Experiment Videos

Last Updated: Jan 8, 2026

Purification of the Dendritic Filopodia-rich Fraction
11:51

Purification of the Dendritic Filopodia-rich Fraction

Published on: May 2, 2019

5.6K
Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons
10:13

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons

Published on: February 10, 2016

12.7K
Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila
07:13

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila

Published on: January 7, 2019

14.6K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Mitochondrial Biology

Background:

  • Mitochondrial dynamics, including fission and fusion, are vital for neuronal health and function.
  • Mitochondrial morphology differs between neuronal compartments (axon vs. dendrite), suggesting compartment-specific regulation.
  • Previous work identified mitochondrial fission factor (Mff) as key for axonal mitochondrial size, but its role in dendrites is unclear.

Purpose of the Study:

  • To investigate the role of fission 1 (Fis1) in regulating mitochondrial morphology, dynamics, and function specifically within cortical neuron dendrites.
  • To understand how Fis1 influences the balance of mitochondrial fission and fusion in different neuronal compartments.

Main Methods:

  • Knockdown of Fis1 in cortical neurons using both primary cell culture and in vivo models.
  • Analysis of mitochondrial morphology and dynamics using microscopy.
  • Assessment of mitochondrial function, including membrane potential, calcium handling, and ATP production.
  • Evaluation of neuronal structure, including dendritic branching and spine density.

Main Results:

  • Fis1 depletion selectively reduced mitochondrial length in dendrites, without affecting axonal mitochondria.
  • Loss of Fis1 increased mitochondrial motility and dynamics within dendrites.
  • Fis1 deficiency led to reduced mitochondrial membrane potential, increased sensitivity to complex III inhibition, and impaired calcium uptake.
  • Altered mitochondrial function resulted in elevated resting calcium, increased dendritic branching, and decreased spine density.

Conclusions:

  • Fis1 plays a dendrite-selective role in regulating mitochondrial morphology and dynamics.
  • Fis1 activity is critical for maintaining mitochondrial function, including calcium handling, in neuronal dendrites.
  • Fis1 influences neuronal structure and connectivity by modulating mitochondrial features within dendrites.