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Basic Science and Pathogenesis
Geisa Rodrigues Salles1, Luiza de Andrade Giraldi1, Marimelia Aparecida Porcionatto2,3
1Universidade do Vale do Paraíba, São José dos Campos, São Paulo, Brazil.
Alzheimer'S & Dementia : the Journal of the Alzheimer'S Association
|December 24, 2025
Summary
This study developed a 3D human neuronal model for Alzheimer's disease (AD) using SH-SY5Y cell spheroids. Oxidative stress induced nuclear abnormalities and morphological disarrangement, highlighting the model's potential for AD research.
Area of Science:
- Neuroscience
- Cell Biology
- Biotechnology
Background:
- Three-dimensional (3D) spheroids offer a promising in vitro model for studying neurobiological events.
- These models reduce animal use and replicate key neuronal mechanisms relevant to Alzheimer's disease (AD), such as oxidative stress.
- This study focused on developing a 3D human neuronal model for AD research.
Purpose of the Study:
- To create a 3D human neuronal model of Alzheimer's disease (AD).
- To investigate the effects of oxidative stress on neuronal spheroids.
- To establish a model for studying AD-related cellular dynamics and potential therapeutics.
Main Methods:
- Human neuroblastoma (SH-SY5Y) cells were cultured in non-adhesive microwells to form spheroids.
- Spheroids underwent differentiation for neuronal phenotype development.
- Oxidative stress was induced using hydrogen peroxide (H2O2) to mimic AD conditions.
Main Results:
- Well-structured spheroids were successfully formed and characterized.
- Spheroid area increased initially, then stabilized with differentiation, indicating neuronal commitment.
- Oxidative stress induced nuclear abnormalities and morphological disorganization in the spheroids.
Conclusions:
- The developed 3D neuronal spheroids represent a viable model for AD research.
- This model can be utilized for exploring therapeutic strategies.
- It aids in elucidating complex cellular dynamics and pathways involved in AD pathogenesis.
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