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Updated: Jan 8, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Glycoside Fragmentation in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Natural Products of
18-1-1 Seikadai, Seika-cho, Soraku-gun, Kyoto 619-0284, Japan.
Abstract:
I investigated the tandem mass spectrometry (MS/MS) fragmentation of ginsenoside glycosides using matrix-assisted laser desorption/ionization MS for ginsenosides Rg1, Rh1, Rb1, and Rb3, focusing on their sodium adduct molecules [M+Na]+. The glycosidic linkage at the C-20 position cleaved more readily than those at C-3 and C-6. These glycosides fragmented on their glucosyl acceptor sides, exhibiting C- and Z-type fragmentation, although generally B/Y-type fragment ions are dominant in MS/MS spectra of neutral oligosaccharides. These results suggest that, due to the hydrophobic triterpene skeleton of the aglycone, sodium cations cannot effectively coordinate with the aglycone moiety.
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