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Vitamin K1 Induced Cytotoxic Effects and Transcriptomic Analysis in Jurkat T Lymphocyte Leukemia Cells
Ying Shang1, Shaoyan Si1, Yaya Qin1
1Department of Research, The Ninth Medical Center, Chinese PLA General Hospital, Beijing, People's Republic of China.
Cancer Management and Research
|December 24, 2025
Summary
Vitamin K1 (VK1) exhibits anticancer properties by reducing Jurkat T lymphocyte leukemia cell viability and inducing apoptosis. VK1 also alters gene expression, up-regulating HMGCR and HMGCS1 in these leukemia cells.
Area of Science:
- Hematologic Malignancies Research
- Cancer Cell Biology
- Molecular Oncology
Background:
- Vitamin K1 (VK1) demonstrates anticancer potential in various cancer types.
- Limited data exists on VK1's impact on hematologic malignancies.
Purpose of the Study:
- To assess VK1's cytotoxic effects on Jurkat T Lymphocyte Leukemia Cells (Jurkat T cells).
- To investigate VK1-induced gene expression alterations in Jurkat T cells.
Main Methods:
- Jurkat T cells and normal PBMCs treated with VK1; viability assessed via MTT assay.
- Apoptosis detected using Annexin V-FITC; cell cycle analyzed by PI staining and flow cytometry.
- RNA sequencing identified differentially expressed genes; HMGCR and HMGCS1 mRNA levels confirmed by RT-qPCR.
Main Results:
- VK1 significantly inhibited Jurkat T cell proliferation with no effect on normal PBMCs.
- VK1 induced apoptosis and G0/G1 cell cycle arrest in Jurkat T cells.
- Transcriptome analysis revealed 21 downregulated and 34 upregulated genes; VK1 confirmed to upregulate HMGCR and HMGCS1 mRNA.
Conclusions:
- VK1 exerts cytotoxic effects and modulates gene expression in Jurkat T cells.
- The HMGCR and HMGCS1 gene pathways are implicated in VK1's effects on leukemia cells.
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