Related Experiment Video
Updated: Jan 8, 2026

Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia
Published on: September 18, 2013
Vitamin K1 Induced Cytotoxic Effects and Transcriptomic Analysis in Jurkat T Lymphocyte Leukemia Cells
Ying Shang1, Shaoyan Si1, Yaya Qin1
1Department of Research, The Ninth Medical Center, Chinese PLA General Hospital, Beijing, People's Republic of China.
Purpose:
Vitamin K1 (VK1) has been proved to have anticancer properties in various cancer cells. However, little is known about the effects of VK1 on hematologic malignancies. The aim of this study was to evaluate the cytotoxic effects of VK1 on Jurkat T Lymphocyte Leukemia Cells (Jurkat T cells), as well as to investigate the changes in gene expression.
Methods:
Jurkat T cells and normal human peripheral blood mononuclear cells (PBMCs) were treated with VK1 at different concentrations, and the cell viabilities were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell apoptosis was detected by Annexin V-FITC cell apoptosis detection kit and the cell cycle was determined by PI staining and flow cytometric analysis. Differentially expressed genes (DEGs) of Jurkat T cells induced by VK1 were analyzed by RNA sequencing. The mRNA expression of HMGCR and HMGCS1 were further verified by real-time RT-qPCR.
Results:
VK1 showed an obviously antiproliferative effect on Jurkat T cells but no any effects on normal human PBMCs. VK1 induced Jurkat T cells apoptosis and cell cycle arrest at G0/G1 phase. A total of 21 down-regulated and 34 up-regulated genes were identified by transcriptome analysis. We selected HMGCR and HMGCS1 genes among the significantly up-regulated genes for further RT-qPCR analyzation. It was confirmed that VK1 up-regulated the mRNA expression of HMGCR and HMGCS1.
Conclusion:
VK1 has cytotoxic effects and transcriptional regulation of multiple genes on Jurkat T cells. The genes of HMGCR and HMGCS1 related pathways may play roles in this process.
More Related Videos
Related Concept Videos
Cytotoxic T Cells-mediated Immune Response
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...
Inhibition of Cdk Activity

