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Diversity of the exopolysaccharide cluster I in the Bradyrhizobium genus
Sachiko Masuda1, Ken Shirasu1, Yasuyuki Kawaharada2
1RIKEN Center for Sustainable Resource Science, RIKEN-TRIP, Yokohama, Kanagawa 230-0045, Japan.
Abstract:
Bradyrhizobium, the largest rhizobial genus, is characterized by a variety of exopolysaccharide (EPS) components, such as penta- and tetrasaccharides, depending on the species. However, several genes involved in EPS synthesis remain unknown. In this study, we investigated whether 186 Bradyrhizobium strains possess homologous genes in the EPS cluster I, which is responsible for the synthesis of a pentasaccharide EPS by B. diazoefficiens USDA110. The absence of homologous genes in the B. elkanii and Photosynthetic Bradyrhizobium supergroups, in contrast to the B. japonicum supergroup, suggests that these lineages may utilize distinct and uncharacterized genes involved in tetrasaccharide EPS biosynthesis.
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