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Updated: Jul 29, 2026
Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
Concentration Imbalance-Driven Nucleotide Circuit for Tick-Borne Encephalitis Virus Detection via
Wenfang Wang1, Nan Liu2, Yicheng Zhao1,3
1State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, College of Basic Medical Science, Jilin University, Changchun 130061, China.
Abstract:
Tick-borne encephalitis virus (TBEV) is a severe neurotropic Flavivirus that requires early and sensitive detection. We developed a concentration imbalance-driven nucleotide circuit (CIDNC) combined with DNA-templated silver nanoclusters (DNA-AgNCs) for enzyme-free and direct RNA detection. The system converts trace TBEV cDNA or RNA into fluorescence signals via strand displacement under isothermal conditions. It achieved detection limits of 0.023 nM for cDNA and 0.035 nM for RNA, with single-base mismatch discrimination. The assay successfully detected viral RNA from plasma and lysed virions. With rapid response (25 min), high recovery (97-98%), and no enzyme requirement, this portable and programmable CIDNC system offers a promising, low-cost diagnostic strategy for TBEV and other RNA viruses, especially in resource-limited settings.
