Genetic Mapping and Functional Characterisation of GhCUP Regulating Leaf Curling in Cotton

Area of Science:

• Plant genetics
• Molecular biology
• Agricultural science

Background:

• Leaf size and shape significantly impact photosynthesis and plant architecture.
• Moderate leaf curling can enhance light penetration and ventilation within a plant canopy.

Purpose of the Study:

• To investigate the cytological characteristics of a cotton mutant with cup-shaped leaves.
• To identify the gene responsible for the upward leaf edge curling phenotype in cotton.

Main Methods:

• Genetic analysis to map the causative gene.
• Comparative analysis of gene annotation and expression between mutant and wild-type cotton.
• Virus-induced gene silencing and gene overexpression experiments.
• Promoter analysis to identify cis-element mutations and transcription factor interactions.

Main Results:

• A single incomplete dominant gene, GhCUP, was identified as the cause of cup-shaped leaves.
• GhCUP was mapped to chromosome A11 and identified as GH_A11G3479.
• Mutations in GhCUP's promoter, including a KAN1 binding site, were found in the mutant, affecting gene expression and leading to the cup-shaped phenotype.

Conclusions:

• GhCUP is a key regulator of cotton leaf edge curling.
• Understanding GhCUP's function provides a target for manipulating cotton leaf shape.
• This research offers potential for optimizing cotton canopy structure to enhance photosynthesis and crop yield.