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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Electrospray Ionization (ESI) Mass Spectrometry01:12

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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Mass Spectrometry: Overview01:19

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Ion Mobility-Mass Spectrometry Imaging: Advances in Biomedical Research.

Mengya Liu1, Chi Zhang1, Lili Xu1

  • 1Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-Ku, Hamamatsu 431-3192, Shizuoka, Japan.

Biotech (Basel (Switzerland))
|December 24, 2025
PubMed
Summary

Ion mobility-mass spectrometry imaging (IM-MSI) combines molecular imaging with structural analysis for enhanced biomarker discovery. This technique offers new insights into diseases like cancer and neurological disorders.

Keywords:
biomedical applicationsion mobility–mass spectrometry (IM-MS)ion mobility–mass spectrometry imaging (IM-MSI)isomerismmass spectrometry imaging (MSI)

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Area of Science:

  • Biomedical science
  • Analytical chemistry
  • Molecular imaging

Background:

  • Mass spectrometry imaging (MSI) maps biomolecule distribution in tissues.
  • Ion mobility-mass spectrometry (IM-MS) characterizes molecules by collision cross-section (CCS).
  • Integrated ion mobility-mass spectrometry imaging (IM-MSI) combines spatial mapping with structural information.

Purpose of the Study:

  • To summarize the principles and classifications of IM-MSI.
  • To review the biomedical applications of IM-MSI.
  • To discuss data processing workflows and tools for IM-MSI.

Main Methods:

  • Coupling ion mobility (IM) separation with mass spectrometry imaging (MSI).
  • Utilizing collision cross-section (CCS) for isomer separation.
  • Applying IM-MSI in oncology and neuropsychiatric disorder research.

Main Results:

  • IM-MSI provides both spatial distribution and structural characteristics of biomolecules.
  • This technique enhances biomarker discovery and understanding of disease mechanisms.
  • IM-MSI offers unprecedented insights in oncology and neuropsychiatric disorders.

Conclusions:

  • IM-MSI is a powerful integrated workflow for molecular analysis.
  • It advances biomarker discovery and disease mechanism research.
  • The review covers IM-MSI principles, applications, and data processing.