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An Improved Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method with Reduced

Warisara Sretapunya1, Thitiya Buranachat1, Montita Prasomthong1

  • 1Nakhon Nayok Hospital, Nakhon Nayok 26000, Thailand.

Biosensors
|December 24, 2025
PubMed
Summary

A new, cost-effective immunochromatography system simplifies dengue virus (DENV) serotyping. This rapid detection method enhances clinical and epidemiological studies by providing accurate DENV serotype identification.

Keywords:
NS1dengue virusimmunochromatographyrapid testserotype discrimination

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Area of Science:

  • Virology
  • Immunology
  • Medical Diagnostics

Background:

  • Dengue virus (DENV) has four serotypes (DENV-1 to DENV-4) with significant protein homology.
  • Current DENV serotyping via RT-PCR is time-consuming and expensive.
  • Rapid, low-cost detection systems are needed for effective dengue management.

Purpose of the Study:

  • To develop an improved, user-friendly immunochromatography system for rapid DENV serotyping.
  • To enhance a previous prototype by integrating multiple serotype detections into single devices.
  • To reduce sample volume and simplify handling for clinical applications.

Main Methods:

  • Development of a two-device lateral-flow cassette system, combining DENV-1/DENV-2 and DENV-3/DENV-4 detection.
  • Optimization of sample buffers for single-buffer compatibility across all four DENV serotypes.
  • Evaluation of the new system using laboratory and clinical DENV isolates and patient specimens.

Main Results:

  • The improved system successfully integrated DENV serotype detection into fewer devices.
  • A single sample buffer was sufficient for all four serotypes, simplifying device handling.
  • The new system demonstrated comparable sensitivity to the previous version with 100% serotype specificity.

Conclusions:

  • The enhanced immunochromatographic assay offers a rapid, cost-effective, and user-friendly method for DENV serotyping.
  • This improved diagnostic tool is suitable for clinical settings, aiding in timely patient diagnosis.
  • The system is expected to accelerate both prospective and retrospective epidemiological research on dengue fever.