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  5. Agricultural, Veterinary And Food Sciences
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  11. Rapid On-site Detection Of Zearalenone In Maize Using A Colloidal Gold Immunochromatographic Strip

Rapid On-Site Detection of Zearalenone in Maize Using a Colloidal Gold Immunochromatographic Strip

Mengjiao Wu1,2,3, Xiaofei Hu3, Lu Fan3

  • 1College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China.

Biosensors
|December 24, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

A new colloidal gold assay rapidly detects zearalenone (ZEN), a mycotoxin in maize, ensuring food safety. This sensitive test strip offers a reliable on-site screening method for consumers and regulators.

Area of Science:

  • Food Science
  • Analytical Chemistry
  • Immunotechnology

Background:

  • Zearalenone (ZEN) is a stable mycotoxin with estrogenic activity produced by *Fusarium* species, posing a significant food safety risk.
  • Accurate and rapid detection methods are crucial for monitoring ZEN contamination in food products like maize.

Purpose of the Study:

  • To develop a rapid, sensitive, and on-site detection method for zearalenone (ZEN) in maize.
  • To establish a colloidal gold immunochromatographic assay (CG-ICA) for reliable ZEN screening.

Main Methods:

  • ZEN was derivatized and conjugated to a carrier protein to produce a monoclonal antibody.
  • The antibody was conjugated to colloidal gold nanoparticles (AuNPs) for use in a CG-ICA test strip.
  • Optimization of assay parameters including pH, antigen concentration, antibody dosage, and incubation time was performed.
Keywords:
colloidal gold immunochromatographic assay (CG-ICA)food safetyzearalenone (ZEN)

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Main Results:

  • The optimized CG-ICA strip demonstrated a detection limit of 11.79 pg/mL and an IC50 of 99.06 pg/mL.
  • A linear detection range of 13.40-732.48 pg/mL was achieved with excellent specificity.
  • Recoveries in spiked maize samples ranged from 85.36% to 98.86% with RSDs below 10%.

Conclusions:

  • The developed CG-ICA strip is a rapid, sensitive, and robust tool for on-site ZEN screening in maize.
  • This assay platform can be adapted for detecting other food contaminants by utilizing different antibodies.