Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

749
Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
749
Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers01:19

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

516
Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
516

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determining the age cut-off for increased surgical risk after Roux-en-Y gastric bypass in elderly patients: MBSAQIP 2015-2023 database analysis.

Surgical endoscopy·2026
Same author

Microcystic lymphatic malformation of the small intestine presenting as acute abdominal pain in a 5-year-old boy: a case report and review of the literature.

Journal of medical case reports·2026
Same author

Practical synthesis and photophysical characterization of full-color circularly polarized luminescent 4,7-diaryl-5,6-(<i>R</i>/<i>S</i>)-BINOL-<i>O</i>-[2,1,3]benzothiadiazoles.

RSC advances·2026
Same author

Comparison of one anastomosis gastric bypass to standard bariatric interventions: a propensity score matching analysis using the 2020-2023 Metabolic and Bariatric Surgery Accreditation and Quality Improvement Program database.

Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery·2026
Same author

Hydration-stable PVA-based skin phantom for wearable biopotential sensor evaluation.

Scientific reports·2026
Same author

Desiccation Tolerance and Persistence of Salmonella enterica and Cronobacter sakazakii on Food Contact Surfaces Based on Cell State and Relative Humidity.

Journal of food protection·2026
Same journal

Unveiling the procoagulant state in Alzheimer's disease: A novel PET imaging strategy.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Estimated labor market outcomes of people progressing from preclinical to early-stage Alzheimer's disease in the United States.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Amyloid exacerbates tau and alpha-synuclein pathologies, behavioral impairments, and neuroinflammation in a mixed dementia model.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Multimorbidity burden and patterns associated with DeepBrainNet-derived brain-age gap in dementia-free older adults: A community-based study.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Reply to "Shifting the emphasis of brain health literacy from individuals to systems to reduce inequalities".

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Shifting the emphasis of brain health literacy from individuals to systems to reduce inequalities.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
See all related articles

Related Experiment Video

Updated: Jan 7, 2026

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies
07:20

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Published on: January 28, 2014

37.1K

Biomarkers.

Robert Lin1, Hien Nguyen1, Jason Wan1

  • 1Taudia, PALO ALTO, CA, USA.

Alzheimer'S & Dementia : the Journal of the Alzheimer'S Association
|December 24, 2025
PubMed
Summary
This summary is machine-generated.

We developed a novel diagnostic platform for Alzheimer's Disease (AD) using dual-conjugation proximity ligation assay (dcPLA). This automated system offers a faster, less invasive method for detecting key biomarkers like pTau-217, improving patient outcomes.

More Related Videos

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances
07:35

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Published on: October 11, 2018

7.9K
Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans
08:14

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

702

Related Experiment Videos

Last Updated: Jan 7, 2026

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies
07:20

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Published on: January 28, 2014

37.1K
Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances
07:35

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Published on: October 11, 2018

7.9K
Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans
08:14

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

702

Area of Science:

  • Neuroscience
  • Biomarker Discovery
  • Diagnostic Technology

Background:

  • Blood-based biomarkers (BBMs) offer advantages over traditional methods like PET and CSF testing for Alzheimer's Disease (AD).
  • Phospho-tau 217 (pTau-217) is a highly specific and clinically relevant biomarker for AD.
  • A need exists for a sample-to-answer, multiplexing diagnostic platform for neurodegenerative diseases.

Purpose of the Study:

  • To introduce the first sample-to-answer multiplexing diagnostic platform specifically designed for Alzheimer's Disease (AD).
  • To address the gap in market availability for automated neurodegeneration diagnostic platforms.

Main Methods:

  • Development of a novel antibody modification approach combined with a bead-based assay to enhance Proximity Ligation Assay (PLA) sensitivity and specificity.
  • Implementation of a patented dual-conjugation PLA (dcPLA) strategy, simplifying workflow and enabling direct integration with sample-to-answer systems.
  • Demonstration of platform adaptability for menu expansion with other antibodies.

Main Results:

  • Detection and quantification of endogenous pTau-217 levels in human plasma samples using dcPLA with high consistency and signal-to-noise ratio.
  • Successful demonstration of a fully automated, sample-to-answer diagnostic platform with a streamlined workflow.
  • The platform processes up to 48 samples in approximately five hours with no hands-on time post-loading, showing near 100% recovery in automated spike and recovery experiments.

Conclusions:

  • Presentation of the first AD-focused diagnostic platform integrating an FDA-approved sample-to-answer system with dcPLA technology.
  • The platform enables minimally invasive and scalable testing, potentially aiding drug developers in patient recruitment for clinical trials.
  • The technology holds promise for advancing early AD detection, longitudinal monitoring, and personalized treatment strategies.