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Updated: Jan 7, 2026

A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement
Published on: February 22, 2022
Physiological, Performance, and Oxidative Stress Responses to High-Intensity Uphill and Downhill Interval Training
George Theofilidis1, Gregory C Bogdanis2, Antonia Kaltsatou1
1Experimental Physiology & Myology and Lifestyle Medicine Laboratory, CREHP, DPESS, School of Physical Education Sports Science and Nutrition, University of Thessaly, Karyes, 42100 Trikala, Greece.
High-intensity interval training on different slopes affects aerobic performance. Uphill running enhances lactate tolerance, while downhill running improves running economy and maximal aerobic speed.
Area of Science:
- Exercise Physiology
- Sports Science
- Biochemistry
Background:
- Interval training is a popular method for improving aerobic performance.
- The effects of incline on training adaptations are not fully understood.
- Oxidative stress markers may be influenced by exercise intensity and duration.
Purpose of the Study:
- To investigate the impact of opposing running slopes on aerobic performance and redox determinants.
- To compare the effects of uphill versus downhill interval training.
- To assess changes in maximal oxygen uptake, running economy, and oxidative stress markers.
Main Methods:
- Fourteen volunteers completed 8 weeks of high-intensity interval training (HIIT) on either a +10% (uphill) or -10% (downhill) grade.
- Training involved ten 30-second runs at 90% of maximal aerobic speed (MAS) with a 1:2 work-to-rest ratio.
- Evaluated parameters included VO2max, MAS, running economy, time to exhaustion, blood lactate, and redox markers (TAC, PCs).
Main Results:
- VO2max remained unchanged in both groups.
- Maximal aerobic speed (MAS) increased in the downhill group.
- Uphill interval training led to increased blood lactate levels, suggesting enhanced tolerance, while downhill training showed improvements in running economy.
Conclusions:
- Both uphill and downhill interval training maintained VO2max levels.
- Uphill HIIT may enhance lactate tolerance, while downhill intermittent running may improve running economy.
- Divergent changes in metabolic, redox, and performance indicators were observed between the two training modalities.
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