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Determination of the Glycogen Content in Cyanobacteria
Published on: July 17, 2017
Pyrrolidine Alkaloids from Mangrove Fungus Penicillium sp. DM27 Enhance L6 Cell Glucose Uptake
Feng-Kai Fan1, Wen-Ting Zhang1,2, Philomina Panin Edjah2
1Hubei Key Laboratory of Purification and Application of Plant Anticancer Active Ingredients, School of Chemistry and Life Science, Hubei University of Education, Wuhan 430205, China.
Abstract:
Ten previously undescribed pyrrolidine alkaloids, namely penicipyrrolidines O-X (1-10), were isolated from the mangrove-derived fungus Penicillium sp. DM27, along with five known compounds (11-15). Their structures were determined by comprehensive analysis of HRESIMS and NMR spectroscopic data, and the absolute configurations were established based on biosynthetic considerations and TDDFT-ECD calculations. All isolates were evaluated for their glucose uptake capacity. Notably, penicipyrrolidine P (2) significantly enhanced cellular glucose uptake in L6 myotubes by 3.83-fold, demonstrating activity comparable to that of metformin, whereas penicipyrrolidines Q and R (3 and 4) showed relatively weaker effects.
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