Related Experiment Video

Updated: Jan 7, 2026

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

1.0K

CRISPR-mRNA synergy: toward adaptive cancer immunotherapy

Ozgur Tanriverdi1

  • 1Department of Medical Oncology & Molecular Biology and Genetics, Mugla Sitki Kocman University Faculty of Medicine, Mugla, Türkiye.

Expert Review of Anticancer Therapy
|December 24, 2025
PubMed
Abstract

Insights

Combining CRISPR genome editing with mRNA cancer vaccines enhances tumor antigenicity and immune response for adaptable cancer immunotherapy. This integration shows promise for personalized cancer treatment, with ongoing research addressing delivery and safety.

Area of Science:

  • Oncology
  • Immunotherapy
  • Genome Editing
  • Vaccinology

Background:

  • CRISPR genome editing and mRNA vaccine technologies offer novel approaches for cancer immunotherapy.
  • Their combination may enhance tumor antigenicity and enable rapid, tailored immune responses.

Purpose of the Study:

  • To review how CRISPR-mediated modulation of cancer pathways and immune responses can improve mRNA neoantigen vaccine efficacy.
  • To evaluate preclinical and clinical studies on CRISPR editing, mRNA cancer vaccines, and combination strategies.

Main Methods:

  • Structured literature search of PubMed, Web of Science, and Scopus (2013-2025).
  • Inclusion of preclinical and clinical studies on CRISPR editing, mRNA cancer vaccines, and integrated strategies.

Main Results:

  • CRISPR-driven tumor sensitization amplifies T-cell responses to mRNA vaccination in preclinical models.
  • Early-phase clinical trials indicate feasibility and durable immune activity with sequential CRISPR editing and mRNA vaccination for melanoma, NSCLC, and pancreatic cancer.

Conclusions:

  • CRISPR-mRNA integration offers a promising strategy for adaptive, evolution-aware oncology.
  • Advancements in delivery and regulation may establish this combination as a cornerstone of future personalized cancer therapy.
Keywords:
AI-driven medicineCRISPRcancer gene therapyimmunotherapymRNA vaccineprecision oncology

More Related Videos

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

1.0K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.8K

Related Concept Videos

Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
6.9K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.6K
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.4K
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.3K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K
Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
5.8K