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Silence of circPLK1 inhibits NSCLC progression by regulating the miR-1294/SLC16A9 axis

Chuankui Li1, Yifan Yang1, Qicai Li1

  • 1Department of Thoracic Surgery, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui 233000, PR China.

Molecular Immunology
|December 24, 2025
PubMed
Abstract

Insights

Circular RNA polo-like kinase 1 (circPLK1) promotes non-small cell lung cancer (NSCLC) progression. Silencing circPLK1 inhibits NSCLC cell proliferation, migration, and invasion via the miR-1294/SLC16A9 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality globally.
  • Circular RNAs (circRNAs) are implicated in cancer progression, but their specific roles require elucidation.
  • The function of circRNA polo-like kinase 1 (circPLK1) in NSCLC pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the role and mechanism of circPLK1 in non-small cell lung cancer (NSCLC).
  • To determine the regulatory relationship between circPLK1, miR-1294, and SLC16A9 in NSCLC.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure circPLK1 expression.
  • Bioinformatics analysis to predict miRNA-target interactions.
  • Cellular assays (CCK-8, colony formation, flow cytometry, Transwell) to assess proliferation, viability, apoptosis, migration, and invasion.
  • Western blotting for protein analysis and xenograft models for in vivo validation.

Main Results:

  • circPLK1 expression was significantly upregulated in NSCLC cells.
  • Silencing circPLK1 inhibited NSCLC cell viability, proliferation, migration, and invasion, while promoting apoptosis.
  • circPLK1 functions as a molecular sponge for miR-1294, enhancing SLC16A9 expression.
  • Knockdown of circPLK1 suppressed tumor growth in vivo by modulating the miR-1294/SLC16A9 axis.

Conclusions:

  • circPLK1 plays a crucial role in promoting NSCLC progression.
  • circPLK1 inhibition suppresses NSCLC proliferation, migration, and invasion through the miR-1294/SLC16A9 pathway.
  • circPLK1 represents a potential therapeutic target for NSCLC treatment.
Keywords:
Non-small cell lung cancerProliferationSLC16A9circPLK1miR-1294

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