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Updated: Jan 7, 2026
Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
Published on: May 5, 2022
Pluripotent stem cells-based neural organoids for modelling human brain development and diseases
Lingling Tong1, Peiqi Tian1,2, Ruoxi Wang1,2
1Center for Reproductive Medicine of The Second Affiliated Hospital, Center for Regeneration and Cell Therapy of Zhejiang, University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, Zhejiang University, Hangzhou, 310003, Zhejiang, China.
Brain organoids offer advanced human neurodevelopment models, overcoming rodent limitations. These complex systems aid in understanding brain disorders and accelerating therapeutic discovery for precision neuroscience.
Area of Science:
- Neuroscience
- Developmental Biology
- Genetics
Background:
- Traditional rodent models have limitations in studying human neurodevelopment and brain disorders.
- Brain organoids provide a transformative in vitro platform for human neuroscience research.
- Evolutionary, cellular, and molecular perspectives are crucial for understanding brain complexity.
Purpose of the Study:
- To systematically compare neural development in mice and humans.
- To highlight the evolution and advancements in brain organoid technologies.
- To explore the applications of organoids in disease modeling and drug discovery.
Main Methods:
- Comprehensive literature analysis synthesizing evolutionary, cellular, and molecular data.
- Detailed examination of organoid technology evolution from basic to advanced systems (e.g., vascularized, assembloids).
- Integration of multi-omics approaches (transcriptomics, epigenomics, proteomics) for organoid validation.
Main Results:
- Organoid technology has advanced significantly, enabling recapitulation of inter-regional connectivity and in vivo fidelity.
- Multi-omics integration validates organoid accuracy and reveals novel disease mechanisms.
- Organoids are effective in modeling neurodevelopmental disorders and facilitating drug screening.
Conclusions:
- Brain organoids bridge translational gaps, offering patient-specific disease modeling and accelerating therapeutic discovery.
- These human-relevant platforms overcome species limitations in neuroscience research.
- Ongoing challenges include vascularization, functional maturation, and ethical considerations, but organoids promise precision neuroscience.
