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Related Experiment Video

Updated: Jan 7, 2026

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

A Chip-Based Surveillance Platform for Detecting Antibody-Based Immunity and Immune Escape Using SARS-CoV-2

Maisha M Feroz^1,2, Seok-Joon Kwon^1,2, Katherin Berman³

¹Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York, USA.

Biotechnology and Bioengineering

|December 25, 2025

Summary

A new chip-based platform enables rapid, large-scale screening of population immunity against viral threats like SARS-CoV-2. This technology can monitor immune escape from emerging virus variants, aiding in vaccine and treatment development.

Keywords:

High‐throughput screening SARS‐CoV‐2 biochip human cell culture immune escape population surveillance pseudovirus viral immunity

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Last Updated: Jan 7, 2026

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Published on: January 12, 2024

Area of Science:

Virology
Immunology
Biotechnology

Background:

Viral diseases like COVID-19, influenza, and RSV necessitate effective treatments and vaccines.
Emerging viral strains pose a risk of immune evasion, complicating public health efforts.
Current population screening methods for viral immunity are often slow, inefficient, and costly.

Purpose of the Study:

To demonstrate a high-throughput, chip-based platform for scalable population immune surveillance.
To assess the platform's ability to screen for immunity and immune escape against SARS-CoV-2 variants.
To evaluate the effectiveness of patient sera and dried blood spot eluates against different SARS-CoV-2 strains.

Main Methods:

Development and utilization of a chip-based platform for high-throughput screening.
Testing antibodies against SARS-CoV-2 (Wuhan-Hu-1 strain) pseudovirus surrogates.
Screening patient-derived sera and eluates from dried blood spots against SARS-CoV-2 pseudoviruses.

Main Results:

The chip-based platform successfully screened for antibodies against SARS-CoV-2 spike proteins.
Patient sera and dried blood spot eluates showed inhibitory activity against the Wuhan-Hu-1 strain.
Some samples demonstrated cross-reactivity, inhibiting infection by the Omicron BA.4/5 variant, indicating potential against distinct spike proteins.

Conclusions:

The demonstrated chip-based platform is scalable for population-wide immune surveillance.
This technology can monitor existing immunity and detect immune escape from emerging viral variants.
The platform supports the development of effective treatments and vaccines against rapidly evolving viral pathogens.