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Updated: Jul 10, 2026

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Published on: April 29, 2007

BEADS: An Interactive Semi-Automated Workflow for 3D Fibrin Angiogenesis Assays Enabling Co-Culture and

Cailin R Gonyea¹, Brielle Hayward-Piatkovskyi², Jason P Gleghorn^1,2

¹Department of Biomedical Engineering, University of Delaware, Newark, DE, USA.

Biorxiv : the Preprint Server for Biology

|December 25, 2025

Summary

A new semiautomated image analysis tool, Bead-based Endothelial Angiogenesis Data Suite (BEADS), quantifies endothelial sprouting in 3D fibrin angiogenesis assays. BEADS significantly reduces analysis time while enabling detailed spatial and co-culture assessments.

Keywords:

3D fibrin bead assay Angiogenesis Endothelial co-culture Semi-automated analysis Sprout directionality microvascular imaging

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Last Updated: Jul 10, 2026

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

Published on: April 29, 2007

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Published on: February 16, 2018

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

Published on: September 18, 2019

Area of Science:

* Biomedical Engineering
* Cell Biology
* Quantitative Imaging

Background:

* 3D fibrin bead angiogenesis assays are crucial for studying endothelial sprouting in vitro.
* Existing analytical methods are time-consuming and struggle with complex imaging, hindering quantitative analysis of co-cultures and spatial interactions.
* There is a need for standardized, efficient tools to analyze angiogenesis in complex experimental setups.

Purpose of the Study:

* To develop a semiautomated, user-interactive image analysis pipeline named Bead-based Endothelial Angiogenesis Data Suite (BEADS).
* To provide standardized, quantitative, bead-centric metrics for sprouting, migration, and spatial orientation in 3D fibrin angiogenesis assays.
* To enable advanced analyses including co-culture designation, nearest-neighbor pairing, and sprout directionality.

Main Methods:

* Integrated automated bead detection with manual correction for accuracy.
* Employed guided annotation of sprouts and migratory cells across multi-channel image z-stacks.
* Incorporated novel capabilities for co-culture identification, nearest-neighbor analysis, and circular statistics for directionality.

Main Results:

* BEADS reduced hands-on analysis time approximately sevenfold compared to manual tracing.
* Sprout-length accuracy was preserved against manual ground truth.
* Demonstrated performance in assays with co-cultured human pulmonary microvascular endothelial cells (HPMECs).

Conclusions:

* BEADS offers a standardized, extensible platform for microvascular image analysis.
* The tool supports co-culture experiments, spatial endothelial interaction metrics, and migratory cell quantification.
* This semiautomated workflow enhances quantitative microvascular research by combining computational precision with biological insights.