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Related Experiment Video

Updated: Jan 7, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Cell Barcoding Reveals Lineage-dependent Outcomes in hiPSC Cardiac Differentiation.

Sogu Sohn¹, Daylin Morgan¹, Cody Callahan¹

¹Department of Biomedical Engineering, The University of Texas at Austin.

Biorxiv : the Preprint Server for Biology

|December 25, 2025

Summary

This summary is machine-generated.

Clinical scalability of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) can be improved by controlling clonal heterogeneity. Tracking hiPSC lineages revealed that specific lineages preferentially differentiate into cardiomyocytes, impacting outcomes.

Keywords:

Cell Differentiation Cell Lineage Induced Pluripotent Stem Cells Tissue Engineering

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Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

Area of Science:

Stem Cell Biology
Cardiovascular Research
Biotechnology

Background:

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) show promise for cardiovascular disease treatment.
Clinical translation is hindered by poor scalability and significant heterogeneity in hiPSC-CM differentiation.
Controlling hiPSC clonal heterogeneity is a potential strategy to enhance scalability.

Purpose of the Study:

To investigate the phenomenon of "fate priming" in hiPSC cardiac differentiation.
To determine if clonal lineage identity influences differentiation outcomes.
To assess the impact of clonal heterogeneity on hiPSC-CM production.

Main Methods:

Utilized the ClonMapper cell barcoding platform for labeling, tracking, and isolating distinct hiPSC lineages.
Compared cardiac differentiation outcomes between single-clone hiPSC populations and heterogeneous multi-clone populations.
Analyzed lineage-specific differentiation preferences towards cardiomyocytes or non-cardiomyocytes.

Main Results:

Identified specific hiPSC lineages exhibiting preferential differentiation into either hiPSC-CMs or non-cardiomyocytes.
Demonstrated significant differences in cardiac differentiation outcomes between isolated single-clone populations and heterogeneous populations.
Confirmed the influence of lineage identity on hiPSC cardiac differentiation.

Conclusions:

hiPSC clonal heterogeneity significantly impacts cardiac differentiation efficiency and outcomes.
The concept of "fate priming" is relevant to hiPSC cardiac differentiation.
Targeting and controlling hiPSC lineage identity can improve the clinical scalability of hiPSC-CMs.