Effect of osmotic stabilizers on 14 CO 2 production by bacteria and blood

Evolution of (14)CO(2) by whole blood as well as by Diplococcus pneumoniae, Haemophilus sp., Pseudomonas aeruginosa, Pseudomonas diminuta, and Streptococcus pyogenes was examined by using the BACTEC system. The control medium was JLI no. 6A culture vial containing 30 ml of enriched tryptic soy broth and 1.5 muCi of (14)C-substrate. Hypertonic media consisted of control medium with either 1 or 3% NaCl, 10% sucrose, and 5%, 10%, or 15% dextran. The most deleterious treatment to bacteria was 3% NaCl since it not only retarded (14)CO(2) production, but also prevented growth of D. pneumoniae, Haemophilus, and P. diminuta. The 10% sucrose treatment diminished (14)CO(2) output, although it did not retard growth of test organisms. This effect was probably due to (14)C-substrate dilution rather than to osmotic effects. Dextran had slight effect on (14)CO(2) production and slightly acidified the medium. Of the treatments tested, only 10% sucrose reduced normal output of (14)CO(2) by whole blood. This also is probably due to (14)C-substrate dilution. It appears that 10% sucrose is potentially the most useful osmotic agent for radiometric techniques since, although bacterial (14)CO(2) production is lowered, blood (14)CO(2) is lowered also.