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Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
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An Accurate Size-Probability Distribution Method for Converting Microplastic Counts to Mass.

Hongyu Chen1,2,3, Jianguo Tao1,2,3, Teng Wang4

  • 1School of Geography and Ocean Science, Nanjing University, Nanjing 210093, China.

Environmental Science & Technology
|December 25, 2025
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Summary

Estimating microplastic (MP) mass is crucial for environmental studies. A new size-probability distribution method accurately calculates MP mass from particle counts, offering a reliable alternative when detailed data is missing.

Keywords:
Yangtze River Basinmicroplastic pollutionmonitoring methodologyparticle size distributionvolume-density conversion

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Polymer Science

Background:

  • Microplastic (MP) mass is vital for environmental fate and transport studies.
  • Current methods for estimating MP mass are limited, especially without detailed morphological data.

Purpose of the Study:

  • To develop and validate a reliable method for estimating microplastic mass using particle count data.
  • To integrate size distribution characteristics with volume-density models for accurate mass quantification.

Main Methods:

  • A size-probability distribution method was developed, combining a conditional fragmentation distribution (CFD)-based size model with volume approximations.
  • The method's optimal configuration was determined by evaluating against measured mass data from balance and mass spectrometry.
  • Performance was compared against coefficient-based conversion and direct volume-density calculations.

Main Results:

  • The proposed size-probability distribution method demonstrated superior performance compared to coefficient-based conversion approaches.
  • The method achieved accuracy comparable to direct volume-density calculations.
  • Application to Yangtze River data yielded annual microplastic mass fluxes between 1950.00 and 12,655.58 tons (mean 6895.90 ± 3763.24 tons).

Conclusions:

  • The developed method offers a reliable and efficient approach for estimating microplastic mass from particle count data.
  • It provides accurate and comparable mass estimates across various microplastic size classes.
  • This technique enhances the understanding of microplastic pollution dynamics in aquatic environments.