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Updated: Jan 7, 2026

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples
Published on: June 6, 2025
Nationwide whole-genome sequencing reveals local transmission of Mycobacterium tuberculosis in Poland, 2003-2020
Alina Minias1, Lidia Fiedorowicz2,3, Monika Kozińska4
1Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland. aminias@cbm.pan.pl.
Background:
Tuberculosis (TB) remains highly prevalent in many countries and is a significant contributor to infectious disease mortality. Combating TB requires monitoring the global circulation of strains, and analyzing the prevalence of specific strains within populations. Currently, whole-genome sequencing (WGS) enables the precise tracking of TB transmission. Here, we utilized spoligotyping combined with whole-genome sequencing (WGS) to identify cases of M. tuberculosis transmission in Poland. Our study aimed to identify M. tuberculosis clones transmitting in Poland before the beginning of the war in Ukraine and the influx of refugees.
Methods:
We used spoligotyping data to identify M. tuberculosis isolates that were possibly transmitted. We used WGS to confirm transmission, and to identify transmission chains.
Results:
A total of 163 isolates were successfully sequenced with high-quality genomic data. Two significant clusters were identified: a pan-susceptible Euro-American lineage cluster (WGS-Cluster 3, n = 32, 19.6%) associated with patients experiencing homelessness and centered in Bydgoszcz (2006-2014); and a multidrug-resistant Beijing lineage cluster (WGS-Cluster 13, n = 32, 19.6%) emerging between 2016 and 2020 across five cities.
Conclusion:
M. tuberculosis transmission should be monitored with routine WGS for better TB control in Poland.
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