Histone phosphorylation analysis of two main TCM syndromes of chronic fatigue syndrome

This study aimed to investigate differences in protein expression, protein function, and epigenetic modifications related to histone phosphorylation in peripheral blood mononuclear cells among patients with chronic fatigue syndrome (CFS) presenting two common Traditional Chinese Medicine (TCM) syndrome types compared with healthy controls.

Three groups of participants were selected from the Medical Examination Center of the Affiliated Hospital of Shandong University of Traditional Chinese Medicine between January 2024 and December 2024. The first group consisted of 10 CFS patients with the Qi and Blood Deficiency syndrome pattern; the second group included 10 CFS patients with the Liver Depression and Spleen Deficiency syndrome pattern; and the third group comprised 10 healthy controls. Phosphopeptide enrichment analysis was employed to detect and identify differentially expressed proteins, followed by Gene Ontology (GO) enrichment analysis.

Differences were observed between both TCM syndrome types of CFS (Qi and Blood Deficiency, Liver Depression and Spleen Deficiency) and the healthy control group in various biological processes and cellular components, including intracellular signal transduction, actin filament bundling, and cortical cytoskeleton organization.

Multiple processes, structures, and functions-such as intracellular signaling, presynaptic modulation of chemical synaptic transmission, actin filament bundling, cortical cytoskeleton organization, SH3 domain binding, and profilin binding-are associated with the occurrence and development of CFS.