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Updated: Jun 20, 2026

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes

Published on: August 16, 2018

Innovative Membrane Purification of Plant-Based Lipase from Flaxseed Using Graphene Oxide.

Nicole Novelli do Nascimento¹, Paulo Waldir Tardioli², Rosângela Bergamasco³

¹Postgraduate Program in Food Science, Centre of Agrarian Sciences, State University of Maringá, Av. Colombo, 5790, CEP 87020-900 Maringá, PR, Brazil.

Journal of Agricultural and Food Chemistry

|December 26, 2025

Summary

This study optimized flaxseed lipase extraction using a novel membrane. The enhanced purification method significantly boosted enzyme activity, making it a sustainable industrial biocatalyst.

Keywords:

alternative purification biocatalytic processes flaxseed byproduct lipase modified membrane technology

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Area of Science:

Biocatalysis
Enzyme Technology
Sustainable Chemistry

Background:

Plant-derived lipases are cost-effective and sustainable alternatives to microbial/animal enzymes.
Flaxseed exhibits high lipase activity during germination, but efficient extraction is difficult.
Developing efficient purification methods is crucial for industrial applications.

Purpose of the Study:

To optimize extraction and purification strategies for active lipase from germinated, defatted flaxseed.
To evaluate a novel membrane-based purification system for enhanced lipase recovery and activity.
To assess the stability and reusability of the purified flaxseed lipase.

Main Methods:

Lipase extraction from flaxseed germinated for an optimized duration (4 hours).
Purification using a membrane modified with polyethylenimine (PEI) and tannic acid (TA)-functionalized graphene oxide (GO) nanoparticles.
Characterization of membrane properties (pore size, antifouling) and lipase thermal stability.

Main Results:

Optimized germination at 4 hours yielded maximum specific activity.
The PEI/TA-GO modified membrane increased lipase specific activity by approximately 11.6-fold.
The membrane exhibited reduced pore size, improved antifouling properties, and allowed reuse for at least two cycles with 74.38% flow recovery.
Purified lipase showed good thermal stability between 25 and 60 °C.

Conclusions:

The developed membrane-based approach enables efficient production of high-activity flaxseed lipase.
This method enhances the sustainability and economic viability of flaxseed lipase as an industrial biocatalyst.
Flaxseed lipase shows significant potential for various industrial applications due to its improved extraction and stability.