Ag Nanoparticle Arrays for Highly Sensitive and Reliable Sulfide Detection
Jiahao Pan1, Xiangting Hu1, Xing Xing1
1College of Engineering and Applied Sciences, Nanjing University, Nanjing 210093, China.
Chemical & Biomedical Imaging
|December 26, 2025
Summary
This study presents a silver nanoarray sensor for sensitive hydrogen sulfide (H2S) detection. The nanoarray offers reliable, ultrasensitive biological sensing for early disease diagnosis.
Area of Science:
- Nanotechnology
- Biosensing
- Analytical Chemistry
Background:
- Early disease diagnosis requires sensitive detection of signaling molecules like hydrogen sulfide (H2S).
- H2S is crucial in cardiovascular and neurological signal transduction.
- On-chip immunoanalysis using nanoarrays enables ultrasensitive detection.
Purpose of the Study:
- To develop a silver nanoparticle (NP) array for sensitive sulfide detection.
- To leverage the reactivity of silver with sulfide for signal generation.
- To establish a reliable platform for biological sensing.
Main Methods:
- Fabrication of a silver nanoparticle (NP) array.
- Utilizing the reaction between Ag and S²⁻ to form Ag₂S, causing a decrease in scattering intensity.
- Implementing parallel reactions on the nanoarray for enhanced reliability.
Main Results:
- The silver nanoarray sensor demonstrated high sensitivity to hydrogen sulfide.
- Detection achieved across a wide dynamic range of 7 orders of magnitude (10 fM to 10 nM).
- The parallel array design significantly improved measurement reliability.
Conclusions:
- The developed Ag nanoarray sensor provides an advanced platform for ultrasensitive sulfide detection.
- This approach offers enhanced sensitivity for biological sensing applications.
- The platform holds potential for detecting various other biosignaling molecules.
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