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High-throughput Measurement of Gut Transit Time Using Larval Zebrafish
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Optimizing blue poo: A validated, cost-effective method for measuring whole gut transit time.

Cyra Schmandt1,2, Julia Trunz1,3, Claudio Perret1,3

  • 1Swiss Paraplegic Research, Nottwil, 6207, Switzerland.

Methodsx
|December 26, 2025
PubMed
Summary

This study optimized the blue dye method for measuring whole gut transit time (WGTT), offering an affordable and minimally invasive alternative. A standardized protocol using a capsule with dye, rice crackers, and liquid ensures accurate WGTT assessment.

Keywords:
Blue dye methodDigestionGastrointestinal diseaseGastrointestinal tractValidation study

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Area of Science:

  • Gastroenterology
  • Pharmacokinetics
  • Clinical Diagnostics

Background:

  • Whole gut transit time (WGTT) is crucial for assessing gastrointestinal health.
  • Traditional WGTT methods are often costly or invasive, limiting their widespread use.
  • A need exists for affordable, minimally invasive WGTT measurement techniques.

Purpose of the Study:

  • To optimize and validate the blue dye method for WGTT measurement.
  • To establish a standardized, practical, and accurate protocol for WGTT assessment.
  • To evaluate different delivery methods and dye concentrations for optimal WGTT determination.

Main Methods:

  • The study utilized "Hollinger Farbpulver Blau" (food colors E131, E132) at concentrations from 30 mg to 241 mg.
  • Four delivery methods were tested: capsule with liquid, gummy bear, muffin, and capsule with rice crackers and liquid.
  • WGTT was measured across various delivery methods and dye concentrations to identify limitations and optimal parameters.

Main Results:

  • Different delivery methods and dye concentrations yielded WGTTs ranging from 18 to 29 hours.
  • Capsules with liquid showed inconsistent transit times, gummy bears caused staining, and muffins were perishable.
  • The optimal protocol involved a capsule with 60 mg of dye, taken with rice crackers and liquid, ensuring accuracy and convenience.

Conclusions:

  • The standardized blue dye method offers a valid, affordable, and minimally invasive approach to WGTT measurement.
  • This optimized method is suitable for large-scale population studies and clinical applications.
  • The established protocol provides reliable and reproducible WGTT measurements.