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Related Experiment Video

Updated: Jan 7, 2026

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model
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LncRNA DANCR Activates p38/mTOR-Mediated Autophagy via ANXA2 to Exacerbate Oxygen-Induced Retinal Neovascularization

Yini Wang1,2, Jiale Bai1,2, Shihong Zhao1,2,3,4

  • 1Aier Academy of Ophthalmology, Central South University, Changsha, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|December 26, 2025
PubMed
Summary

Long noncoding RNA DANCR promotes retinal neovascularization by activating autophagy via the Annexin A2/p38 MAPK/mTOR pathway. Targeting this pathway offers a novel therapeutic strategy for retinopathy of prematurity (ROP).

Keywords:
DANCRannexin A2autophagyretinal neovascularizationretinopathy of prematurity

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Last Updated: Jan 7, 2026

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model
10:32

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

Published on: April 2, 2021

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Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Genetics

Background:

  • Retinal neovascular diseases, such as retinopathy of prematurity (ROP), share pathological similarities with oxygen-induced retinopathy (OIR) mouse models.
  • Long noncoding RNAs (lncRNAs) are implicated in retinal neovascularization, but their precise functions are not fully understood.

Purpose of the Study:

  • To investigate the role of lncRNA DANCR in retinopathy of prematurity (ROP) and its underlying molecular mechanisms.
  • To explore DANCR as a potential therapeutic target for ROP.

Main Methods:

  • Utilized oxygen-induced retinopathy (OIR) mouse models and human retinal microvascular endothelial cells (ECs).
  • Assessed the effects of DANCR overexpression and knockdown on EC proliferation, migration, apoptosis, and angiogenesis.
  • Employed RNA pull-down and RNA immunoprecipitation assays to identify binding partners of DANCR.
  • Investigated the involvement of the ANXA2/p38 MAPK/mTOR pathway.
  • Administered intravitreal RNA interference targeting DANCR in OIR mice.

Main Results:

  • lncRNA DANCR was upregulated in ROP models.
  • DANCR overexpression promoted EC proliferation, migration, and angiogenesis while suppressing apoptosis through autophagy activation.
  • DANCR directly bound to Annexin A2 (ANXA2).
  • ANXA2 knockdown partially reversed DANCR-induced pro-angiogenic effects by modulating the p38 MAPK/mTOR pathway.
  • Intravitreal inhibition of DANCR reduced ANXA2 expression, ameliorated retinal pathology, and improved visual function in OIR mice.

Conclusions:

  • lncRNA DANCR exacerbates retinal neovascularization in OIR models by activating autophagy via the ANXA2/p38 MAPK/mTOR axis.
  • The DANCR/ANXA2/p38 MAPK/mTOR pathway represents a novel therapeutic target for treating ROP.