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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
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Related Experiment Video

Updated: Jan 7, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
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Published on: January 15, 2015

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Fine-Tuning Lipid-Coated Porous Silicon Nanoparticles for siRNA Delivery.

Pouya Dehghankelishadi1, Parisa Badiee1, Shiying Qiao1

  • 1Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, Australia.

Advanced Healthcare Materials
|December 26, 2025
PubMed
Summary

This study refines porous silicon nanoparticles (pSiNPs) for cancer siRNA delivery. New lipid formulations show improved tumor accumulation in mice, offering a promising, well-tolerated cancer treatment strategy.

Keywords:
breast cancerlipid coatingporous silicon nanoparticlessmall interfering RNA

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Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
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Area of Science:

  • Biotechnology and Nanomedicine
  • Cancer Therapeutics
  • Drug Delivery Systems

Background:

  • Small interfering RNA (siRNA) holds promise for cancer treatment but faces delivery challenges.
  • Porous silicon nanoparticles (pSiNPs) offer versatile nanoengineering for siRNA delivery.
  • Previous pSiNP formulations utilized calcium silicate sealing and lipid coating.

Purpose of the Study:

  • To refine pSiNP-based siRNA delivery systems by investigating alternative lipid compositions.
  • To compare ionisable lipids (MC3) against quaternary ammonium lipids (DOTAP) for siRNA loading, cellular association, and in vivo performance.
  • To evaluate the efficacy of antibody functionalization (aDSG2) for enhanced transfection.

Main Methods:

  • Formulation of pSiNPs with calcium silicate sealing and lipid coatings (MC3 and DOTAP).
  • In vitro assessment of siRNA loading, cellular association, lysosomal escape, and transfection efficiency.
  • In vivo biodistribution studies in an orthotopic breast cancer murine model.
  • Evaluation of formulation tolerability at a 20 mg/kg dose.
  • Functionalization of lipid-coated pSiNPs with anti-desmoglein-2 antibody (aDSG2).

Main Results:

  • MC3 formulation showed lower siRNA loading and in vitro cellular association than DOTAP.
  • Comparable lysosomal escape and acceptable siRNA transfection were observed for MC3.
  • MC3 formulation demonstrated higher tumor accumulation in vivo.
  • Formulations were well-tolerated at the tested dose.
  • Antibody functionalization aimed to improve transfection efficacy.

Conclusions:

  • Ionizable lipid (MC3) coated pSiNPs enhance tumor accumulation compared to DOTAP, despite lower in vitro loading, suggesting potential for improved cancer therapy.
  • The developed pSiNP formulations are well-tolerated and demonstrate potential for targeted siRNA delivery.
  • Further refinement via antibody functionalization may improve transfection efficiency for enhanced therapeutic outcomes.