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Related Experiment Video

Updated: Jan 7, 2026

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

Low-Cost Open Platform Digital Light Printer (OP-DLP) for 96-Well Format Hydrogel Printing and Localized

Katelyn Mathis^1,2, Afia Ibnat Kohon^1,2, Natanael Monroy¹

¹Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, Texas 76207, United States.

ACS Biomaterials Science & Engineering

|December 26, 2025

Summary

Researchers developed an open-platform digital light printer (OP-DLP) for versatile light-based surface processing. This adaptable device enables efficient hydrogel synthesis and precise biomolecule activation in multiwell formats.

Keywords:

biomaterials devices hydrogel printing light-activation

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Last Updated: Jan 7, 2026

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

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Published on: December 26, 2010

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Published on: March 31, 2022

Area of Science:

Biomaterials Science
Surface Chemistry
Photochemistry

Background:

High-throughput light-based surface processing is crucial for applications like hydrogel printing and biomolecule activation.
Existing methods often lack flexibility, require complex synthesis, or are not suitable for multiwell plate formats.

Purpose of the Study:

To introduce an open-platform digital light printer (OP-DLP) for versatile 2D hydrogel synthesis and spatial biomolecule activation.
To address limitations of current devices in terms of flexibility, ease of use, and multiwell plate compatibility.

Main Methods:

Development of an open-platform digital light printer (OP-DLP) with a LabVIEW interface for controlled printing settings and planar corrections.
Demonstration of OP-DLP's capability for hydrogel printing in a 96-well format with precise thickness control.
Showcasing spatial activation of photocaged DNA on a surface using the OP-DLP system.

Main Results:

OP-DLP successfully produced hydrogel layers with precise thickness and consistent results across a 96-well plate.
The device demonstrated the ability to form 2D gels with specific shapes and modified ink compositions in different wells.
Localized de-caging of photocaged DNA on a surface was achieved, highlighting the spatial activation capability.

Conclusions:

The OP-DLP offers a flexible and accessible solution for light-based surface processing, suitable for diverse research applications.
Its open-platform design allows for customization with different wavelengths and printing vessels, enhancing its utility.
OP-DLP facilitates high-throughput hydrogel synthesis and precise spatial control over biomolecule activation in multiwell formats.