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Updated: Jan 7, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
CNOT1 contributes to small nuclear non-coding RNA maturation
Chisato Umehara1, Reika Sakurai1, Hiroaki Sako2
1Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo, 160-8582, Japan.
Abstract:
CCR4-NOT complex is involved in transcription, translation, and mRNA degradation. As of yet, it is unknown if CCR4-NOT has additional functions beyond those. It is possible to predict unknown functions of genes by using co-expressed network analysis. Here we show that all CCR4-NOT subunits highly co-express with modulators of small nuclear noncoding RNA biogenesis, predicting that CCR4-NOT regulates the maturation of small nuclear noncoding RNA. In agreement with this, knockdown of CNOT1 decreased the expression levels of small nuclear non-coding RNAs independent of the canonical function of CCR4-NOT complex. However, the reduced expression was rescued by the co-suppression of RBM7, mediating exosomal degradation of misprocessed small RNA. These results suggest that CNOT1 contributes to small nuclear non-coding RNA maturation.
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