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Updated: Jun 19, 2026
Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
Published on: March 18, 2014
LED blue light photobiomodulation induces ferroptosis and apoptosis via ROS-mediated oxidative damage in osteosarcoma
Jiali Yang1, Haokuan Qin2, Xiaojing Miao3
1Jiangsu Institute of Marine Resources Development, Jiangsu Ocean University, Lianyungang 222005, China; School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China; School of Information Science and Technology, Fudan University, Shanghai 200438, China.
Blue light photobiomodulation (PBM) using LEDs shows tumor-suppressive effects on osteosarcoma cells. This non-pharmacological approach induces cell cycle arrest, apoptosis, and ferroptosis, offering a promising therapeutic avenue.
Area of Science:
- Oncology
- Biophotonics
- Cell Biology
Background:
- Blue light photobiomodulation (PBM) demonstrates therapeutic potential in various conditions, including cancer.
- The specific effects of LED blue light PBM on osteosarcoma cells and its molecular mechanisms are not well-understood.
Purpose of the Study:
- To investigate the tumor-suppressive effects of LED blue light PBM on human osteosarcoma cell lines (HOS and MG63).
- To elucidate the underlying molecular mechanisms of blue light PBM in osteosarcoma.
Main Methods:
- Treatment of HOS and MG63 osteosarcoma cell lines with LED blue light photobiomodulation.
- Assessment of cell proliferation, cell cycle progression, apoptosis, and ferroptosis.
- Analysis of key ferroptosis-associated proteins (HO-1, PTGS2, GPX4) and oxidative stress markers (MDA, LPO).
Main Results:
- Blue light PBM significantly suppressed osteosarcoma cell proliferation and induced G2/M phase cell cycle arrest.
- PBM treatment activated both apoptosis and ferroptosis pathways, evidenced by altered protein expression.
- Increased reactive oxygen species (ROS) accumulation, indicated by elevated MDA and LPO levels, confirmed ferroptosis induction and oxidative stress.
Conclusions:
- LED blue light PBM exhibits significant tumor-suppressive properties against human osteosarcoma cells.
- The mechanism involves inducing cell cycle arrest, apoptosis, and ferroptosis via ROS-mediated oxidative stress.
- Blue light PBM represents a promising non-pharmacological therapeutic strategy for osteosarcoma, meriting further clinical research.
