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Chlormequat chloride-responsive VviDOF3.4-VviLBD36 module regulates adventitious root formation in grapevine
Rui Yu1, Pengwei Jing1, Jiayin Niu1
1College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, 471023, Henan Province, China; Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization, Luoyang, 471023, Henan Province, China.
Plant Physiology and Biochemistry : PPB
|December 27, 2025
Summary
Chlormequat chloride (CCC) enhances adventitious root (AR) formation in grapevines. This effect is mediated by the VviDOF3.4 transcription factor activating VviLBD36, promoting AR development.
Area of Science:
- Plant Biology
- Molecular Biology
- Agricultural Science
Background:
- Adventitious root (AR) formation is crucial for grapevine asexual reproduction.
- Chlormequat chloride (CCC), a gibberellin antagonist, regulates plant growth but its role in AR formation is understudied.
Purpose of the Study:
- To investigate the effect of CCC on AR formation in 'Thompson Seedless' grapevines.
- To identify key molecular factors involved in CCC-mediated AR formation.
Main Methods:
- CCC treatment of grapevines
- Transcriptome sequencing of AR
- Gene expression analysis (overexpression in Arabidopsis)
- Yeast one-hybrid and dual-luciferase assays
Main Results:
- CCC treatment significantly promoted AR formation in grapevines.
- VviLBD36 was identified as a key gene upregulated by CCC, promoting AR and lateral root (LR) development.
- VviDOF3.4 was found to directly bind and activate the VviLBD36 promoter, also promoting LR development.
Conclusions:
- The VviDOF3.4-VviLBD36 module plays a critical role in CCC-induced AR formation in grapevines.
- This study provides a molecular basis for understanding CCC's effects on grapevine root development.
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