Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

541
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
541
Biofilms01:29

Biofilms

1.0K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The oral microbiome associated with early childhood caries in preschool children: A scoping review.

Journal of dentistry·2026
Same author

Exploring the associations between dietary nitrate and nitrite intake and markers of self-reported periodontal disease in the UK Biobank cohort.

European journal of nutrition·2026
Same author

38% silver diamine fluoride for dentine hypersensitivity treatment: an <i>in situ</i> study.

Frontiers in oral health·2026
Same author

Staging-dependent dysbiosis of plaque microbiota in early childhood caries.

Journal of dentistry·2026
Same author

Managing dentine erosion with silver diamine fluoride: An in situ study.

Journal of dentistry·2026
Same author

Arresting root caries with nonrestorative strategies: A systematic review with network meta-analysis.

Journal of dentistry·2026

Related Experiment Video

Updated: Jan 7, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
10:42

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

17.8K

High-resolution microbial changes in root caries revealed by Type IIB Restriction-site associated DNA for microbiome.

Josie Shizhen Zhang1, Yufeng Zhang1, Shi Huang1

  • 1Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Hong Kong SAR, China.

Journal of Dentistry
|December 27, 2025
PubMed
Summary

Root caries involves specific bacterial shifts, not just diversity changes. High-resolution sequencing revealed distinct species and metabolic pathways in carious root biofilms compared to healthy sites.

Keywords:
Dental cariesDental plaqueHuman microbiomeMetagenomicsMicrobiotaRoot caries

More Related Videos

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
09:44

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Published on: October 20, 2011

16.5K
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
09:55

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

28.0K

Related Experiment Videos

Last Updated: Jan 7, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
10:42

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

17.8K
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
09:44

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Published on: October 20, 2011

16.5K
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
09:55

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

28.0K

Area of Science:

  • Oral microbiology
  • Dental caries research
  • Metagenomics

Background:

  • Root caries is a significant oral health issue, particularly in older adults.
  • Understanding the specific microbial communities associated with root caries is crucial for developing targeted prevention and treatment strategies.

Purpose of the Study:

  • To characterize species-level microbial and functional alterations in dental biofilms of root caries using high-resolution sequencing.
  • To compare the oral microbiome of carious root surfaces with caries-free root surfaces within the same individuals and with healthy controls.

Main Methods:

  • Utilized Type IIB Restriction-site Associated DNA for Microbiome (2bRAD-M) analysis on supragingival plaque samples.
  • Collected samples from older adults with active root caries and healthy controls, from both carious (CC) and caries-free (CH) root surfaces, and caries-free (HH) root surfaces of controls.
  • Employed nonparametric tests to compare microbial diversity, species abundance, and predicted functional pathways.

Main Results:

  • No significant differences in overall microbial diversity were found between groups.
  • Microbial divergence was significantly greater between carious (CC) and caries-free (CH) root microbiota within patients compared to controls (HH).
  • Increased abundance of specific species, including Propionibacterium acidifaciens and Prevotella multisaccharivorax, was observed in carious sites. Nine KEGG pathways, mainly related to carbohydrate metabolism, were positively associated with root caries.

Conclusions:

  • Significant differences exist in the microbiota between carious and caries-free root surfaces at the individual tooth level.
  • Root caries is characterized by shifts in specific microbial species and their metabolic functions, rather than broad diversity changes.
  • Tooth-level resolution is critical for understanding the microbial etiology of root caries.