Related Experiment Video
Updated: Jan 7, 2026
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
Published on: July 6, 2022
Protocol to evaluate mouse brain spatial cell type-resolved transcriptomic discoveries using 10× Visium spatial
Mara S Burns1, Ricardo Miramontes2, John C Reidling2
1Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA.
Abstract:
Understanding changes in gene expression and cell-cell signaling among spatial regions in diseased tissues adds critical biological information to understanding mechanisms. Here, we present a protocol to investigate molecular transcriptional drivers within intact murine tissue using 10× Genomics Visium spatial transcriptomics and 10× Genomics FLEX single-cell RNA sequencing (scRNA-seq) data. We describe steps for collecting mouse brain tissue from multiple ages, processing samples, and mounting the tissue. We then detail procedures for staining, FLEX tissue section collection, fixation, dissociation, and cell storage. For complete details on the use and execution of this protocol, please refer to Burns et al.1.
