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Related Experiment Video

Updated: Jan 7, 2026

Establishing a Mouse Contusion Spinal Cord Injury Model Based on a Minimally Invasive Technique
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Establishing a Mouse Contusion Spinal Cord Injury Model Based on a Minimally Invasive Technique

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Protocol to develop a preclinical severe contusive-compressive SCI mouse model for translational research.

Sissi Dolci1, Marzia Di Chio1, Giuseppe Busetto2

  • 1Laboratory of Pharmacology, Department of Diagnostic and Public Health, University of Verona, 37134 Verona, Italy.

STAR Protocols
|December 28, 2025
PubMed
Summary

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This summary is machine-generated.

This study details a protocol for inducing severe spinal cord injury (SCI) in mice. This reproducible model aids in preclinical testing of novel therapies for neurological damage.

Area of Science:

  • Neuroscience
  • Regenerative Medicine
  • Surgical Techniques

Background:

  • Complete spinal cord injury (SCI) results in irreversible neurological damage.
  • Current therapies for SCI are limited due to failed neural repair.

Purpose of the Study:

  • To present a detailed protocol for inducing severe contusive-compressive SCI at the thoracic T11 level in mice.
  • To establish a reproducible preclinical model for testing SCI therapies.

Main Methods:

  • Laminectomy at the T11 thoracic level.
  • Induction of SCI using the NYU-MASCIS II impactor.
  • Validation of injury through functional and histological analysis.

Main Results:

  • Successful induction of severe contusive-compressive SCI.
Keywords:
Health SciencesModel OrganismsNeuroscience

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  • Demonstration of key aspects of human secondary injury.
  • Establishment of a reliable model for preclinical research.

    • Conclusions:

    • The described protocol provides a valuable tool for the preclinical testing of SCI therapies.
    • This model effectively replicates critical features of human secondary injury, facilitating research into effective treatments.