Direct photoconjugation multi-wall carbon nanotube-based immunosensor platform for clenbuterol determination

Area of Science:

• * Electrochemistry and Nanomaterials Science
• * Biosensor Development and Analytical Chemistry

Background:

• * Clenbuterol is a banned substance in food production due to health risks.
• * Accurate and sensitive detection methods are crucial for food safety and regulatory compliance.
• * Existing detection methods can be complex, costly, or lack sufficient sensitivity.

Purpose of the Study:

• * To develop a selective and cost-effective potentiometric sensor for clenbuterol detection.
• * To utilize a multi-walled carbon nanotube (MWCNT) platform functionalized with anti-clenbuterol antibodies.
• * To achieve label-free and sensitive clenbuterol quantification.

Main Methods:

• * Fabrication of a sensor using anti-clenbuterol antibody immobilized on a photosensitive amino acid-modified MWCNT platform.
• * Utilizing an MWCNT-bound antibody as the indicator electrode and Ag/AgCl as the reference electrode.
• * Potentiometric measurement for clenbuterol detection.

Main Results:

• * The developed immunopotentiometric sensor demonstrated a rapid response time of 1 minute.
• * Achieved a broad linear detection range from 1.0 × 10^-11 M to 1.0 × 10^-5 M.
• * Exhibited a remarkably low detection limit of 4.83 × 10^-12 M with an operational lifetime of 36 weeks at pH 6.0.

Conclusions:

• * The proposed sensor provides a highly selective, sensitive, and cost-effective method for clenbuterol detection.
• * The label-free, potentiometric approach offers advantages for routine clenbuterol monitoring in various matrices.
• * This technology presents a promising tool for ensuring food safety and regulatory adherence.