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Updated: Jul 6, 2026
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A Protocol to Acquire the Degenerative Tenocyte from Humans
Published on: June 9, 2018
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Temporal Gene Expression Changes in Rotator Cuff Tendon Injury and Repair
Eui-Sup Lee1, Jae Hee Choi2, Yu-Na Lee2
1Department of Orthopaedic Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Summary
Researchers identified YAP1 as a key biomarker for rotator cuff tendon degeneration and healing. This finding offers potential for improved assessment and treatment of these common injuries.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Orthopedics
Background:
- Rotator cuff tendon injuries are prevalent, especially in older adults, yet effective biomarkers for degeneration and healing are scarce.
- Current markers lack sensitivity in reflecting dynamic changes during tendon repair processes.
Purpose of the Study:
- To identify dynamic biomarkers for assessing rotator cuff tendon degeneration and healing.
- To investigate temporal gene expression and histological changes in a chronic rotator cuff tear model.
Main Methods:
- Utilized a rat model of chronic rotator cuff injury and repair, analyzing gene expression and histology over time.
- Validated findings using human tendon samples and an in vitro 3D tendon construct model with mechanical loading.
- Examined tendon-specific markers (Scleraxis, Tenascin C, Collagen I, Collagen III) and YAP1 expression.
Main Results:
- Collagen I and YAP1 were downregulated in degeneration and upregulated post-repair, unlike other markers.
- YAP1 re-expression correlated with improved collagen organization during healing.
- YAP1 demonstrated mechanosensitivity, with mechanical loading enhancing its nuclear localization and tendon gene expression in vitro.
- YAP1 was consistently reduced in human degenerative tendons.
Conclusions:
- YAP1 is a promising, mechanosensitive biomarker for rotator cuff tendon degeneration and regeneration.
- YAP1 acts as a mechanotransducer, reflecting tendon remodeling processes.
- Findings highlight YAP1's translational value across animal, human, and in vitro models for therapeutic potential.
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