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Related Concept Videos

Alternative RNA Splicing02:18

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Energy Balance01:19

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The human body gets energy from the three macronutrients: carbohydrates, proteins, and fats. Energy is released when the chemical bonds in the organic compounds present in the food are broken down. The energy content of food is measured in kilocalories (kcal), defined as the amount of heat required to raise the temperature of one kilogram of water by one degree Celsius. This value is determined by measuring the temperature change of the water surrounding a calorimeter after the complete...
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Organisms must balance energy intake with the energy required for growth, maintenance and reproduction. These trade-offs result in a variety of survivorship and reproductive strategies, including semelparity and iteroparity. Semelparous species, like annual plants, have only one reproductive episode in their lifetimes and consequently have short lifespans. Iteroparous species, by contrast, have many reproductive events during their lifetimes but have relatively few offspring. These two...
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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
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Related Experiment Video

Updated: Jan 7, 2026

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans
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Exploring Splicing-Energy Axis Associations to Diet and Longevity.

Stefano Donega1, Myriam Gorospe2, Luigi Ferrucci1

  • 1Translational Gerontology Branch, National Institute on Aging, NIH, Baltimore, Maryland, USA.

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|December 29, 2025
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Summary
This summary is machine-generated.

A low-protein, high-carbohydrate diet extends lifespan in mice by altering RNA splicing, not mitochondrial function. This suggests a new "energy-splicing resilience axis" for healthy longevity.

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Area of Science:

  • Biochemistry
  • Genetics
  • Aging Research

Background:

  • Nutrient composition influences lifespan via mechanisms beyond calorie restriction.
  • Mitochondrial regulation is a known factor in aging, but other pathways are emerging.
  • Dietary interventions are key to understanding aging processes.

Purpose of the Study:

  • To investigate the impact of a low-protein, high-carbohydrate diet on lifespan and cellular mechanisms.
  • To explore the role of RNA splicing in mediating diet-induced longevity.
  • To support the proposed "energy-splicing resilience axis."

Main Methods:

  • Mice were fed a low-protein, high-carbohydrate diet enriched with cellulose.
  • Liver proteome and RNA splicing patterns were analyzed.
  • Connections between nutrient sensing, splicing factors (e.g., SRSF1), and signaling pathways (e.g., AMPK) were examined.

Main Results:

  • The low-protein, high-carbohydrate diet extended lifespan in mice.
  • This lifespan extension was associated with significant shifts in the liver proteome due to altered RNA splicing.
  • The observed effects were distinct from those typically induced by caloric restriction and mitochondrial improvements.

Conclusions:

  • Dietary interventions can promote longevity through RNA splicing modulation, independent of mitochondrial changes.
  • The "energy-splicing resilience axis" provides a framework for understanding how cells adapt to nutritional stress via splicing.
  • Targeting splicing pathways may offer novel strategies for promoting healthy aging and proteome stability.