Related Experiment Video
Updated: Jul 3, 2026
07:07
Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
Published on: January 7, 2019
6.5K
Overexpression of miR-516a-5p Promotes Erosive Oral Lichen Planus: In Vitro Study Based on Bioinformatics Analyses
Yan Chen1, Chen-Xi Li2,3, Rui Xue4
1Division of Pediatric Dentistry and Preventive Dentistry, School/Hospital of Stomatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
Clinical and Experimental Dental Research
|December 29, 2025
Summary
MicroRNA-516a-5p overexpression in erosive oral lichen planus may alter inflammatory cytokines and boost fibroblast proliferation and migration by targeting MAPK11. This finding sheds light on OLP pathogenesis.
Area of Science:
- Molecular Biology
- Immunology
- Cell Biology
Background:
- Erosive oral lichen planus (EOLP) is an inflammatory condition with complex molecular underpinnings.
- MicroRNAs (miRNAs) are key regulators of gene expression implicated in various diseases.
Purpose of the Study:
- To identify differentially expressed miRNAs in EOLP.
- To investigate the functional role of miR-516a-5p in human oral mucosal fibroblasts (OMFs).
Main Methods:
- High-throughput sequencing of miRNA expression profiles from EOLP tissues and healthy controls.
- Bioinformatics analysis to identify enriched pathways and target genes.
- In vitro experiments involving miR-516a-5p overexpression in OMFs.
Main Results:
- 82 miRNAs were differentially expressed in EOLP, primarily associated with T helper cell differentiation and MAPK signaling pathways.
- miR-516a-5p overexpression significantly decreased MAPK11 mRNA levels in OMFs.
- miR-516a-5p altered inflammatory cytokine profiles (increased TNF-α, IFN-γ; decreased IL-4, IL-6, IL-10, IL-13) and enhanced OMF proliferation and migration.
Conclusions:
- miR-516a-5p overexpression in EOLP contributes to T helper 1/2 cell-associated inflammatory cytokine imbalance.
- Targeting MAPK11 mRNA by miR-516a-5p promotes OMF proliferation and migration, potentially influencing EOLP progression.
