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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

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Selective Ag⁺ Sensing and Cellular Imaging Based on Hydrophilic Modulated Peptide Probe.

Shenao Li^1,2, Jieben Jiao^1,2, Shuxian Meng²

¹Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province, Qinghai Minzu University-Zangnuo Joint-Lab, College of Pharmacy, Qinghai Minzu University, Xining, China.

Luminescence : the Journal of Biological and Chemical Luminescence

|December 29, 2025

Summary

Researchers developed new fluorescent probes to detect silver ions (Ag+). One probe, DNSCI-APGYHA, shows high selectivity and sensitivity for silver, enabling effective environmental and cellular imaging.

Keywords:

Ag+ recognition cell imaging nanoscale self‐assembly peptide fluorescent probes

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Published on: September 20, 2016

Area of Science:

Environmental Science
Analytical Chemistry
Biomedical Engineering

Background:

Silver (Ag+) is a toxic heavy metal pollutant with significant environmental and health impacts.
Accurate detection of Ag+ is vital for environmental monitoring and biomedical applications.
Existing peptide fluorescent probes often overlook the self-assembly of probe molecules into nanoparticles.

Purpose of the Study:

To design and synthesize novel Ag+-responsive fluorescent probes by adjusting peptide hydrophilicity.
To investigate the impact of self-assembly on probe performance.
To identify a highly selective and sensitive probe for Ag+ detection.

Main Methods:

Systematic adjustment of peptide hydrophilicity to synthesize three fluorescent probes: DNSCI-SPPW, DNSCI-APGYHA, and DNSCI-GPTHC.
Evaluation of probe selectivity, detection limit, and interference resistance.
Assessment of cell permeability and cytotoxicity for in-cell imaging applications.

Main Results:

DNSCI-APGYHA demonstrated stable morphology, superior selectivity for Ag+, and a low detection limit of 19 nM.
This probe exhibited enhanced resistance to interference compared to others.
DNSCI-APGYHA showed good cell permeability and low cytotoxicity, enabling successful Ag+ imaging in CT26 cells.

Conclusions:

The study highlights the importance of considering molecular self-assembly in designing peptide fluorescent probes.
DNSCI-APGYHA is a promising tool for sensitive and selective detection of Ag+ in complex biological and environmental samples.
The developed probe facilitates advanced applications in cellular imaging and environmental monitoring of silver contamination.