Multimodal Biosensors with Flexible-Concentration Adaptability Based on Single-Component Nanozymes for Highly
Shuai Qin1,2, Yujia Ren1,2, Xuxin Yan1,2
1Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, Shandong 266003, China.
Analytical Chemistry
|December 29, 2025
Summary
This study introduces a novel multimodal biosensor using manganese silicate (MnSiO3) nanoparticles for butyrylcholinesterase (BChE) analysis. This cost-effective platform enables portable BChE detection, inhibitor screening, and disease diagnosis with high accuracy.
Area of Science:
- Biochemistry
- Nanotechnology
- Analytical Chemistry
Background:
- Accurate butyrylcholinesterase (BChE) activity analysis is crucial for physiology and disease diagnosis.
- Existing nanozyme-based biosensors face challenges in synthesis, accuracy, adaptability, and point-of-care application.
- There is a need for versatile, cost-effective single-component nanozymes for BChE analysis and inhibitor screening.
Purpose of the Study:
- To develop versatile multimodal biosensors using economical single-component nanozymes for BChE activity analysis.
- To enable portable detection of BChE, intelligent screening of inhibitors, and potential disease diagnosis.
- To elucidate the catalytic mechanism of MnSiO3 nanoparticles and construct a multi-modal sensing platform.
Main Methods:
- Utilized MnSiO3 nanoparticles as single-component nanozymes with OXD-like activity.
- Employed tandem catalysis involving natural enzymes and MnSiO3 nanozymes.
- Developed a colorimetric-photothermal-fluorescent-RGB quadri-modal platform.
- Applied XNOR-logic-assisted validation for clinical diagnosis and fabricated logic circuits for inhibitor identification.
Main Results:
- Achieved efficient catalytic oxidation of optical substrates by MnSiO3 nanozymes across a broad pH range.
- Demonstrated a versatile quadri-modal platform with flexible concentration adaptability for portable BChE analysis in real samples.
- Successfully performed clinical diagnosis of patients with aberrant hepatocyte function using multimodal synergistic validation.
- Achieved intelligent logical identification of typical inhibitor drugs via concatenated logic circuits.
Conclusions:
- MnSiO3 nanoparticles serve as economical, high-performance single-component nanozymes for advanced biosensing.
- The developed multimodal biosensor platform offers portable, selective, and sensitive analysis of BChE.
- This work provides a novel protocol for logic-encoded smart analysis of drugs and clinical diagnostics.
