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Updated: Jan 7, 2026

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
Published on: December 13, 2014
The Role the Saccharomyces cerevisiae Nonsense-Mediated mRNA Decay Pathway Plays in Metal Toxin Uptake and
Sunday Olaniyan1, Jacqueline Carroll1, Stephnie Nwaiwu1
1Department of Biology, Baylor University, Waco, Texas, USA.
Abstract:
Regulation of gene expression at the messenger RNA (mRNA) level is crucial for organisms to rapidly respond to changing environmental conditions, especially harmful toxins. The highly conserved nonsense-mediated mRNA decay (NMD) pathway is a translation dependent messenger RNA (mRNA) degradation pathway. Although NMD is well known for targeting mRNAs with premature termination codons (PTCs), it is now recognized as a pathway with additional essential regulatory functions. Notably, NMD precisely regulates protein coding natural mRNAs, hence controlling gene expression within several physiologically significant pathways. Pathways controlled by NMD include essential bio-metal homeostasis and metal ion detoxification, and the crosstalk between them. Here, we discuss the relationships between NMD and various metal ion detoxification pathways. We review the role that the NMD pathway plays in cadmium, arsenic, and lithium detoxification.
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