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Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
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Mitochondrial calcium shapes B cell signaling and mitochondrial function.

Hakan Taskiran1,2,3,4, Elena Czeslik1,2, Leonhard Stark2,5

  • 1Institute of Clinical Chemistry and Pathobiochemistry, School of Medicine and Health, University Hospital of Technical University of Munich, Munich, Germany.

Frontiers in Immunology
|December 29, 2025
PubMed
Summary
This summary is machine-generated.

Mitochondrial calcium dynamics in B cells are regulated by the Mitochondrial Calcium Uniporter and impact cell signaling and activity. Understanding this calcium regulation is key to B cell fate and function.

Keywords:
B lymphocytecalciummetabolismmitochondriasignaling

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Calcium (Ca2+) signaling is crucial for B cell activation, differentiation, anergy, and apoptosis.
  • B cell activation involves rapid Ca2+ mobilization from the endoplasmic reticulum and extracellular influx.
  • The role of mitochondrial Ca2+ dynamics in B cells remains largely unexplored.

Purpose of the Study:

  • To investigate the regulation of mitochondrial Ca2+ dynamics in B cells.
  • To determine the functional consequences of altered mitochondrial Ca2+ levels in B cells.
  • To elucidate the role of the Mitochondrial Calcium Uniporter in B cell mitochondrial Ca2+ uptake.

Main Methods:

  • Utilized chemical dyes and a genetically encoded Ca2+ sensor with a mitochondrial targeting sequence.
  • Employed proximity ligation assays to assess endoplasmic reticulum-mitochondria interactions.
  • Studied functional consequences of Mitochondrial Calcium Uniporter loss in primary mouse B cells and Ramos cells.

Main Results:

  • Mitochondrial Ca2+ levels dynamically respond to cell activation, stress, and metabolic cues.
  • Mitochondrial Ca2+ uptake is significantly dependent on the Mitochondrial Calcium Uniporter.
  • Reduced mitochondrial Ca2+ uptake negatively impacts mitochondrial activity and B cell signaling.

Conclusions:

  • Mitochondrial Ca2+ dynamics are integral to B cell responses.
  • The Mitochondrial Calcium Uniporter plays a key role in regulating mitochondrial Ca2+ in B cells.
  • Altered mitochondrial Ca2+ homeostasis affects B cell function and signaling pathways.