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Updated: Jan 7, 2026

An Ex vivo Mast Cell Degranulation Assay using Crude Peritoneal Exudate Cells and Natural Antigen Stimulation
Published on: April 27, 2021
James H Clark1, Zilla Hussain2, Lee Akst1
1Department of Otolaryngology-Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Histamine 2-receptor antagonists (H2RAs) lose efficacy quickly due to tachyphylaxis, with a significant reduction by day 3. This diminished effect, up to 27.5%, suggests H2RAs are not ideal for frequent GERD management.
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